Welcome to send your manuscript to: lifesciencej@gmail.com

No.

Titles / Authors /Abstracts

Full Text

No.

1

Physical Evaluation and In Vitro Bioequivalence Study of Marketed Aspirin Enteric Coated Tablets in KSA

Muhammad Hadi Sultan*, Md Sarfaraz Alam, Osama A. Madkhali, Md Sajid Ali., Muaid Ahmed Aqeel, Ryan Ibrahim Gaderi, Abdullah Muhammad Khardali

College of Pharmacy, Jazan University, Jazan, Saudi Arabia, KSA, Zip Code: 45142

Mobile No.- +966-557119488; E-mails: mhsultan@jazanu.edu.sa, msultan151@gmail.com

Abstract: Purpose: The main objective of this study is to evaluate the in-vitro bio equivalency of enteric coated Aspirin tablets in the Kingdom of Saudi Arabia (KSA). Methods: Two available brands of Aspirin enteric coated tablets were randomly collected from various pharmacies, and were then coded as A (DISPRIN) and B (JUSPRIN). An innovator Aspirin enteric coated tablet was also procured fromalocal community pharmacy. Pure Aspirin drug was procured from Sigma-Aldrich. General quality assessment such as weight variation, hardness, friability, disintegration test, and a dissolution study were performed as per USP guidelines. Results: All A and B brands passed the weight variation test, as no more than two tablets failed, in all cases. The hardness test results for the innovator, and brands A and B, were recorded as 4.91, 5.25 and 4.58 kg, respectively. The friability test was also carried out for the innovator and brands A and B, whereby all experienced weight loss of 0.086%, 0.030% and 0.077%, respectively. Although the results of the disintegration test made it clear that the innovator, as well as brands A and B, did not disintegrate in an acidic medium, all tablets disintegrated in a pH 6.8 buffer solution in 19.36, 21.58 and 15.13 minutes, respectively. There were no significant variations in the dissolution profiles or in the release profiles of the innovator, nor in brands A and B. There was a release of drug in a basic medium within one hour (90.19%, 82.04% and 87.64%, respectively). Finally similarity factors (f₂) were calculated for brands A and B, which were 72.12% and 88.72%, respectively. Conclusion: On the basis of in-vitro tests, brands A and B are considered bioequivalent and interchangeable, while brand B is closer to the innovator in terms of hardness, disintegration and dissolution profile.

[Muhammad Hadi Sultan, Md Sarfaraz Alam, Osama A. Madkhali, Md Sajid Ali., Muaid Ahmed Aqeel, Ryan Ibrahim Gaderi, Abdullah Muhammad Khardali. Physical Evaluation and In Vitro Bioequivalence Study of Marketed Aspirin Enteric Coated Tablets in KSA. Life Sci J 2020;17(9):1-7]. ISSN: 1097-8135 (Print) / ISSN: 2372-613X (Online). http://www.lifesciencesite.com. 1. doi:10.7537/marslsj170920.01.

Key words: Aspirin, Enteric coated tablet, In-vitro bioequivalence, and Dissolution study

Full Text

1

2

Assessment of salt tolerance ability in maize genotypes at seedling stage

Shahid Ali Arshad¹, Aleena Shahid², Tayyaba Mahmood², Faiza Khan², Ali Khan², Samaria Nisar^2*, Muhammad Arif^3*

¹Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Pakistan.

²Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.

³Soil and Water Testing Laboratory, Layyah, Punjab, Pakistan

*Corresponding author’s: arifbodla2015@gmail.com, samariaagrarian@gmail.com

Abstract: Screening of salt tolerant genotypes of agricultural crops is a necessary aspect for future food security. In this study, ten genotypes of maize were used to analyze their performance under saline condition. An experiment was performed as factorial under Completely Randomized Design (CRD) with three replications. At seedling stage all of the genotypes were used for correlation analysis between leaf temperature, chlorophyll contents, shoot fresh weight, shoot dry weight, root fresh weight, and root dry weight. Fresh root length had positive and highly significant correlation with fresh root weight, root density and dry root weight. Root length has negative and highly significant correlation with dry shoot weight. Chlorophyll content had negative and highly significant correlation with leaf temperature. Leaf temperature had negative and highly significant correlation with chlorophyll content and root density but positive and significant correlation was present with dry shoot weight and dry root weight both at genotypic and phenotypic level. Fresh root length had positive and highly significant correlation with fresh root weight, root density and dry root weight. Root length has negative and highly significant correlation with dry shoot weight. Genotypic and phenotypic correlation of fresh shoot weight with fresh root shot ratio, dry root shoot ratio was negative and highly significant. Results presented that fresh shoot weight was positive and in significant correlation with fresh root weight, root density, dry root weight at both genotypic and phenotypic level.

[Arshad SA, Shahid A, Mahmood T, Khan F, Khan A, Nisar A, Arif M. Assessment of salt tolerance ability in maize genotypes at seedling stage. Life Sci J 2020;17(9):8-17]. ISSN: 1097-8135 (Print) / ISSN: 2372-613X (Online). http://www.lifesciencesite.com. 2. doi:10.7537/marslsj170920.02.

Key words: Salinity, maize, correlation analysis, genotypic correlation, phenotypic correlation

Full Text

2

3

A critical review: Carrot and beetroot-based beverages to alleviate hypertension

Poushmal Abdul Razzaq^1*, Adeel Akram¹, Shahida Tahir¹, Adan Ijaz¹, Zain Ul Abedin¹, Iahtisham-Ul-Haq^1,2, Saira Sarwar^1-3, Mafia Akram¹

¹National Institute of Food Science and Technology, University of Agriculture, Faisalabad, Pakistan

²Department of Diet and Nutritional Sciences, Faculty of Health and Allied Sciences, Imperial College of Business Studies, Lahore, Pakistan

³Faculty of Life Sciences, University of Central Punjab, Lahore

*Corresponding author: poushmal.raja@yahoo.com

Abstract: Hypertension is a global epidemic health problem with a huge impact and variation in prevalence across all geographical areas. Hypertension is a leading risk factor for cardiovascular disease, stroke, and chronic renal disease. The incidence of adult hypertension in low- and middle-income countries is far greater than wealthier countries. Moreover, hypertensive adults in developing countries are unable to receive adequate treatment as compared with those in developed countries. Thus, sustainable and local treatments are necessary for adults in low income countries. In this article we critically reviewed the impact of carrot and beetroot-based beverages to alleviate hypertension. The supplementation of carrot and beetroot-based beverages should be promoted as a key component of a healthy lifestyle to control blood pressure in healthy and hypertensive individuals. In conclusion, carrot and beetroot juices supplementation might be an easy, accessible, safe, and evidence-based strategy to alleviate hypertension.

[Razzaq PA, Akram A, Tahir S, Ijaz A, Abedin ZU, Haq IU, Sarwar S, Akram M. A critical review: Carrot and beetroot-based beverages to alleviate hypertension. Life Sci J 2020;17(9):18-28]. ISSN: 1097-8135 (Print) / ISSN: 2372-613X (Online). http://www.lifesciencesite.com. 3. doi:10.7537/marslsj170920.03.

Key words: Hypertension, carrot, beetroot

Full Text

3

4

Gene action and combining ability studies for yield and its component traits in wheat (Triticum aestivum L.)

Samina Sarfraz^1,2*, Noreen Fatima¹, Zainab Saeed¹, Wajeeha Khan¹, Shahid Ali Arshad³, Adila Iram², Hira Saher², Maimona Munir⁴, Misbah Zulfqar^1,5*

¹Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan.

²Rice Research Institute, Kala Shah Kaku, Pakistan

³Institute of Soil and Environmental Sciences, University of Agriculture, Faisalabad, Pakistan.

⁴Government College University, Faisalabad, Pakistan

⁵Oilseeds Research Institute, AARI, Faisalabad, Pakistan

*Corresponding author’s: saminasarfraz19@yahoo.com misbahzulfqar@gmail.com

Abstract: Eight bread wheat (Triticum aestivum L.) genotypes were crossed in a line × tester mating design. The 15 F₁'s and their parents were evaluated in a randomized complete block design with three replications at the research area of Plant Breeding and Genetics, UAF. The characters studied were plant height, spike length, peduncle length, flag leaf area, number of tillers per plant, number of grains per spike, number of spikelet per spike, density of spike, thousand grain weight and yield of grain/ plant at. The results indicated that sufficient genetic variability was observed for all characters studied. Among lines, Aas 2011 proved best general combiner and exhibited significant general combining ability effects for most of the characters. Among testers, 9459 proved best general combiner for most of the traits studied. Among crosses, AARI 2011×9459 and Ujala 2016×9515 were best specific combiners for most of the studied traits and showed highly significant SCA effects. GCA to SCA variances ratio revealed predominantly non-additive gene action for these traits. Development of new varieties with improved agronomic traits is, therefore, suggested through selection in late segregation generations of these crosses.

[Sarfraz S, Fatima N, Saeed Z, Khan W, Arshad SA, Iram A, Saher H, Munir M, Zulfqar M. Gene action and combining ability studies for yield and its component traits in wheat (Triticum aestivum L). Life Sci J 2020;17(9):29-37]. ISSN: 1097-8135 (Print) / ISSN: 2372-613X (Online). http://www.lifesciencesite.com. 4. doi:10.7537/marslsj170920.04.

Key words: Wheat (Triticum aestivum L.), yield, combining ability, Line × tester analysis

Full Text

4

5

Study on the correlation between HO-1 level and white matter lesions of cerebral small vessel encephalopathy

Yusheng Li, Yao Zhang, Shanshan Li, Lulu Yu, Zichen Zhao, Yusheng Li *

Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou 450000, China.

fccliyusheng@zzu.edu.cn

Abstract: OBJECTIVE: Heme oxygenase (HO), a cytoprotective agent has become an ideal and participates in the neuroprotection of various neurological diseases. The purpose of this study is to investigate whether plasma heme oxygenase-1 (HO-1) has role in cerebral small vessel disease (CSVD). METHODS: In this study, there were 147 patients prospectively included with CSVD who were admitted to the three departments of neurology from March 2018 to April 2019, and obtained baseline data, clinical scores, imaging Fazekas scores, and peripheral blood samples of the patients for analysis. According to the patient’s Fazekas score, Fazekas<2 is defined as WMH (-), and Fazekas≥2 is defined as WMH (+) to study the relationship between plasma HO-1 concentration and the degree of white matter lesions. RESULTS: The difference in plasma HO-1 between WMH (+) and WMH (-) patients was not statistically significant (p = 0.082). CONCLUSION: There is no significant correlation between the level of HO-1 and the degree of white matter lesions.

[Yusheng Li, Yao Zhang, Shanshan Li, Lulu Yu, Zichen Zhao, Yusheng Li. Study on the correlation between HO-1 level and white matter lesions of cerebral small vessel encephalopathy. Life Sci J 2020;17(9):38-41]. ISSN: 1097-8135 (Print) / ISSN: 2372-613X (Online). http://www.lifesciencesite.com. 5. doi:10.7537/marslsj170920.05.

Keywords: Cerebral small vessel disease; Biomarker; Cognition; White matter hyperintensity

Full Text

5

6

Supplementation of Gluten-Free Rice Flour with Yeast and Date Powder to Produce Rusk Rich in Selenium and Iron

Amal M. Allbban¹ and Haneen H. Mouminah²

¹Nutrition and Food Science, (applied Nutrition), Umm Al- Qura University, Kingdom of Saudi Arabia

²FOOD and Nutrition Dept., Faculty of Human Science and Design, King Abdulaziz University, Kingdom of Saudi Arabia

Email: amlbban@uqu.ed.sa, hmouminah@kau.ed.sa

Abstract: Bakery products made with wheat flour are not suitable for people with gluten sensitivity or autism, so we have tended to produce rusks from rice flour with yeast and date powder to raise the quality of gluten-free product while remaining highly acceptable to consumers. Chemical, nutritional, physical and sensory characteristics of gluten-free rusks product from rice flour blends with different levels of yeast and date powder were studied. The chemical composition of raw materials and rice flour blends were measured. The results observed that the protein, fat, ash and fiber content were increased with the addition of yeast and dates powder from 5 to 15% levels. The best blends were achieved made from 70% rice flour, 15% yeast and 15% date powder to give rusk contained of 15.30 % protein, 1.43% ash, 2.75 fat and 1.65 fibers, respectively. The results also showed an increase in minerals content of rusk, resulting from increasing of yeast and dates powder. The high proportion of selenium yeast led to an increased selenium and iron rate in the rusk product, which increased from 1.56 and 1.34 mg100/g for rusks made of 90% rice flour, 5 yeast and 5% date powder to 3.77 and 2,06 mg/ 100g for rusks made of 70% rice flour, 15 yeast and 15% date powder. The results also showed that slight decreases in the sensory properties of rusks when increasing the addition of yeast and dates powder. Therefore, it could be recommended that the best sample of rusk was made from 80% and 90% of rice flour to the consumer. Moreover, the results showed a noticeable improvement in the physical properties of rusk with an increase in the ratio of adding yeast and dates powder to rice flour.

[Amal M. Allbban and Haneen H. Mouminah. Supplementation of Gluten-Free Rice Flour with Yeast and Date Powder to Produce Rusk Rich in Selenium and Iron. Life Sci J 2020;17(9):42-50]. ISSN: 1097-8135 (Print) / ISSN: 2372-613X (Online). http://www.lifesciencesite.com. 6. doi:10.7537/marslsj170920.06.

Keywords: Rusk, Rice flour, Date powder, Inactive dry yeast, Selenium, Autism.

Full Text

6

7

Role of MT-1B gene polymorphism in HCC-HCV Egyptian patients

Bothaina Madkour¹, Ahmed R. Mashaal², Mohamed A. Elrefaiy² and Rabab Fouad ¹

¹Department of Hematology, Theodor Bilharz Research Institute, Cairo-Egypt.

²Department of Tropical Medicine, Theodor Bilharz Research Institute, Cairo-Egypt.

dr_rabab_fouad@yahoo.com

Abstract: Background: Metallothioneins (MTs) are a group of metal-binding proteins with a high cysteine content and low molecular weight. MTs have an important role in metal metabolism and protect cells against the toxic effects of radiation, alkylating agents and oxygen free radicals. MT1B polymorphism rs964372 is located in the MT1B intron 1 (C>G) this SNP has great association with many diseases. The frequency of HCC powerfully correlates with liver inflammation like exposure to one or several risk factors including hepatitis B virus (HBV), hepatitis C virus (HCV), inherited metabolic diseases, heavy alcohol abuse, obesity, type 2 diabetes and aflatoxins. As oxidative stress drives genomic damage and genetic instability which cause mutations, and mutations have a crucial role in carcinogeneisis. Methods: 78 HCC patients, 53 HCV infected patients and 61 matched healthy controls were recruited. rs964372 genotypes of MT-1B were assayed with RT-PCR genotyping assays. Results: We found insignificant increase in CG genotype in HCC group (24.4%) compared to control group (23.5 %) and HCV group (22.2%) with higher incidence of C allele in HCV and HCC patients (85.7% and 84% respectively) in comparison to control group (82.4%). Conclusions: rs964372 of MT-1B gene polymorphism is neither related with HCV infection nor with HCC progression. Future studies including large sample size are recommended.

[Bothaina Madkour, Ahmed R. Mashaal, Mohamed A. Elrefaiy and Rabab Fouad. Role of MT-1B gene polymorphism in HCC-HCV Egyptian patients. Life Sci J 2020;17(9):51-56]. ISSN: 1097-8135 (Print) / ISSN: 2372-613X (Online). http://www.lifesciencesite.com. 7. doi:10.7537/marslsj170920.07.

Keywords: hepatocellular carcinoma (HCC), Metallothioneins (MT-1B), Polymerase chain reaction (PCR)

Full Text

7

8

Evaluation of Toxicological Effects of Some Plant Oils and Diesel Oil on Some Biological Aspects of Culex pipiens (Diptera: Culicidae) larvae.

Badriah M. K. Asiri *, Sana Q. Al-Ansari and Nada O. Edrees.

Department of Biology-Zoology, Faculty of Science, Jeddah University, Saudi Arabia.

Abstract: The Culex Pipiens mosquito is considered one of the most important vectors of pathogens to humans, as it transmits malaria and yellow fever to humans all around the world, and due to the harmful effects that traditional chemical pesticides cause on the environment, there was an urgent need to search for natural alternatives to control insects and limit their damage. In this work, the effect of the following treatment with plant oils and diesel oil was studied to compare their effect on some biological aspects of Culex Pipiens mosquitoes. It was found that the value of IC₅₀ for C. limon oil was 9.67, while it was equal to 21.38 ppm in the case of M. piperita oil, as recorded 29.82 ppm for L. angustifolia oil, as the value of IC₅₀ for A. indica oil was 38.06 ppm, while IC₅₀ value was 80.89 ppm when treating mosquito larvae with effective concentrations of diesel oil. The effect on egg production was recorded in the treatments with the four plant oils (C. limon, M. piperita, L. angustifolia, A. indica) and diesel oil respectively 97.4, 102.5, 127.1, 107.2, 124.4 eggs compared to the average number of eggs / female in the control, which recorded 141.6 eggs. These results confirm that the treatment of larvae of C. pipiens with C. limon oil has caused 31.2% a decrease in eggs production in females who lived from the treatments followed by M. piperita oil 24.6%, then A. indica oil 24.3% and L. angustifolia oil 10.2%, while it recorded diesel oil 12.1 % decrease in egg production. These results confirm that the larval treatments with the tested plant oils and diesel oil led to a decrease in the levels of egg hatching in the treatments by about (12.5, 14.7, 5.2, 15.3 and 7.4)%, respectively, compared to the control. These results confirm that the treatment of larvae C. pipiens with the tested oils had a negative effect on longevity in males by (13.4, 10.7, 6.7, 5.4 and 15.4)%, respectively, and in females by (29.5, 20.5, 24.6, 26.9 and 25.7)% on ranking in comparison to average longevity in male and female mosquitoes in the control groups, the results of the statistical analysis revealed that the differences in the average longevity between males resulting from the treatments and the control were not significant, while the differences were significant when comparing the average longevity between the females resulting from the treatments and the females of the control group.

[Badriah M. K. Asiri, Sana Q. Al-Ansari and Nada O. Edrees. Evaluation of Toxicological Effects of Some Plant Oils and Diesel Oil on Some Biological Aspects of Culex pipiens (Diptera: Culicidae) larvae. Life Sci J 2020;17(9):57-70]. ISSN: 1097-8135 (Print) / ISSN: 2372-613X (Online). http://www.lifesciencesite.com. 8. doi:10.7537/marslsj170920.08.

Keywords: Mosquito, Culex pipiens, Plant Oils, larvae, Diesel, pesticide, egg production, Mentha piperita, Lavandula angustifolia, Citrus limon, Azadirachta indica, adult longevity, hatchability.

Full Text

8

9

Therapeutic effect of selenium and vitamin E on arsenic induced cardiac damage in adult male albino rat: Histological, biochemical and pharmacological study

Radwa Mohammed Ahmed¹, Sarwat Lotfi Ahmed Abdel-Latif², Mostafa Yehia Abdelwahed³, Mohamed Hussein Elmahdi⁴, Ayman Mohamed Helal⁵, Heba Hussein Rohym⁶

¹Lecturer, Anatomy and Embryology Department, Faculty of Medicine, Fayoum University, Egypt

²Lecturer, Histology Department, Faculty of Medicine, Fayoum University, Egypt

³Lecturer, Physiology Department, Faculty of Medicine, Fayoum University, Egypt

⁴Lecturer, Pathology Department, Faculty of Medicine, Fayoum University, Egypt

⁵Lecturer, Pharmacology Department, Faculty of Medicine, Fayoum University, Egypt

⁶Lecturer, Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Fayoum University, Egypt

Abstract: Introduction: Arsenic is present naturally in drinking water, soil and air. Exposure to it via drinking water is associated with cardiomyopathy and ischemic heart disease, hypertension, peripheral vascular disease. Selenium and vitamin E co-administration markedlyimproves the deleterious effect of arsenic in rat myocardium. Both agents are natural antioxidants used to decrease tissue inflammatory reaction exerted by free oxygen radicals. Each drug could be used alone in treatment of sodium arsenate induced myocardial damage. However, combined administration of both drugs has a more potent anti-oxidant and antiapoptotic effect compared to using each drug alone. Aim of work: to elucidate the therapeutic role of selenium and vitamin E on sodium arsenate induced histological and biochemical alterations on rat myocardium. Material and methods: Fifty adult male albino rats were divided into five groups, weighing 180-220 g; Group I (Normal control): The rats were injected intraperitoneally once daily with 0.9% saline. Group II (Sodium arsenate administration for two weeks): Therats were injected intraperitoneally with 7.2mg/kg/day of sodium arsenate for two weeks. Group III (Sodium arsenate administration for six weeks): The rats were injected intraperitoneally with 7.2mg /kg/day of sodium arsenatefor six weeks. Group IV (Sodium arsenate for two weeks then selenium +vitamin E co-administration): The rats were injected intraperitoneally with 7.2mg/kg/day of sodium arsenate for two weeksfollowed by co administration with both 10 μg / kg / day selenium, and 20 mg / kg / day vitamin E for another two weeks. Group V (Sodium arsenate for six weeks then selenium +vitamin E co-administration): The rats were injected intraperitoneally with 7.2mg/kg/dayof sodium arsenate for six weeks followed by co administration with both 10 μg / kg / day selenium, and 20 mg / kg / day vitamin E for another two weeks. The heart was excised and processed for the following studies: Light microscopic study with hematoxylin and eosin (H & E) and Masson’s trichrome stains, electron microscopic examination and biochemical estimation of antioxidant enzymes. Results: Examination of specimens of the groupstreated with sodium arsenate revealed disturbed myocardial architecture, nuclear and cytoplasmic degeneration, myocardial fibrosis and marked reduction of tissue levels of antioxidant enzymes. However, selenium –vitamin E co-administration markedly ameliorated these histological and biochemical alterations. Conclusion: It could be concluded that selenium and vitamin E had a beneficial role in treatment of cardiomyopathy caused by sodium arsenate administration in adult male albino rats.

[Radwa Mohammed Ahmed, Sarwat Lotfi Ahmed Abdel-Latif, Mostafa Yehia Abdelwahed, Mohamed Hussein Elmahdi, Ayman Mohamed Helal, Heba Hussein Rohym. Therapeutic effect of selenium and vitamin E on arsenic induced cardiac damage in adult male albino rat: Histological, biochemical and pharmacological study. Life Sci J 2020;17(9):71-83]. ISSN: 1097-8135 (Print) / ISSN: 2372-613X (Online). http://www.lifesciencesite.com. 9. doi:10.7537/marslsj170920.09.

Keywords: Sodium arsenate, Selenium, vitamin E, Heart.

Full Text

9

10

Inter simple sequence repeat (ISSR) markers and some physiological attributes of barley genotypes to drought and potassium nutrition

Samia Ageeb Akladious, Salwa Mohamed Abbas

Biological and Geological Sciences, Faculty of Education, Ain Shams University

El Makres St. Roxy, Cairo, Egypt (ARE) Post code: 1575

samiapola@yahoo.com

Abstract: This study was designed to identify useful effects of potassium for drought tolerance in barley and determine the most tolerant genotypes to drought. Five barley genotypes were grown with two drought stress levels; 50% and 30% field capacity and treated with potassium sulfate in two levels K1 (40) and K2 (80 mg/ kg). Increasing water deficit (30% field capacity) lead to reduce spike length, spike weight, grain number, grain weight as well as 1000 grain weight in Giza 130 and Giza 134 genotypes although using of K1 and K2, whereas both K applications increased yield parameters at 50% field capacity. In Giza 123, 126 and 133 genotypes, applications of K1 and K2 increased yield parameters under 30% and 50% field capacity. Based on the obtained results, Giza 126 genotype showed the highest and stable yield across normal and drought conditions. The epidermis cell number, stomata number and stomata index increased in the upper surfaces of the control leaves in comparison with their lower surfaces. Application of potassium to the drought stressed plants, generally decreased stomata number, stomata index and epidermis cell number. Potassium may have served to adaptation of barley plants to drought stress conditions by causing a decrease in stomata movements. SDS-PAGE analysis has revealed that plant grown under drought showed induction or suppression in the synthesis of few polypeptides. Giza 126 showed best performance in respect of appearance of new bands in protein profile. ISSR-PCR technique was used to detect some molecular markers associated with drought tolerance in the five genotypes. Five ISSR primers were used and revealed 78% polymorphism. The primers produced 12 bands, which could be used as molecular markers and could be useful in breeding programs of barley.

[Samia Ageeb Akladious, Salwa Mohamed Abbas. Inter simple sequence repeat (ISSR) markers and some physiological attributes of barley genotypes to drought and potassium nutrition. Life Sci J 2020;17(9):84-98]. ISSN: 1097-8135 (Print) / ISSN: 2372-613X (Online). http://www.lifesciencesite.com. 10. doi:10.7537/marslsj170920.10.

Keywords: Hordeum vulgare; drought stress; potassium; stomata movements; ISSR markers; SDS-PAGE

Full Text

10