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[Life Sci J 2022;19(4):1-7]. ISSN 1097-8135 (print); ISSN 2372-613X (online). http://www.lifesciencesite.com. 1.

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Construction of retroviral vector pLEGFP-N1-TERT for skin tissue engineering

Ting Tan¹,Weixia Zeng², Zhiqi Hu^3*

¹Department of burn and plastic surgery General Hospital of Nanjing Military Region in Fuzhou, Fujian, China

²Laura Biotech Co., Ltd. Guangzhou, Guangdong Province, China

³Department of Plastic Surgery, Nan Fang Hospital of Southern Medical University, Guangzhou, Guangdong, China

Abstract: Background: The seed cell is the most basic and important factor of tissue engineering. Objective: To construct the retroviral telomerase reverse transcriptase (TERT) and investigate the expression of TERT in neonatal mouse hypodermal cells. Methods: The TERT gene amplified by polymerase chain reaction (PCR) was inserted into plasmid pLEGFP-N1. The positive clone identified by restriction enzyme digestion and sequencing was transfected into packaging cells to produce retrovirus particles. Neonatal mouse hypodermal cells were infected with the virus to generate a stable cell line. The expression of TERT mRNA and enhanced green fluorescent protein (EGFP) was detected by western blot. Results: Retroviral vector pLEGFP-N1-TERT was constructed successfully, and a stable cell line of neonatal mouse hypodermal cells which express EGFP was established. Western blot and immunohistochemical assay showed that the expression level of TERT was significantly elevated in the neonatal mouse hypodermal cells transfection. Conclusion: A high titer of retrovirus TERT mediates high-level expression of the exogenous TERT gene in the neonatal mouse hypodermal cells. Our study has important significance for skin tissue engineering.

[Ting Tan, Weixia Zeng, Zhiqi Hu. Construction of retroviral vector pLEGFP-N1-TERT for skin tissue engineering. Life Sci J 2022;19(4):8-12]. ISSN 1097-8135 (print); ISSN 2372-613X (online). http://www.lifesciencesite.com. 2. doi:10.7537/marslsj190422.02.

Keywords: telomerase reverse transcriptase; retroviral vector; hypodermal cells

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Prediction of Diaphragm Wall Deflection in Deep Excavation Using Evolutionary Fuzzy Neural Inference Model

Min-Yuan Cheng ¹, Yu-Wei Wu ^1*, Hsing-Chih Tsai ¹, Chien-Ho Ko ², Yen-Chuan Lin ¹

^1. Department of Construction Engineering, National Taiwan University of Science and Technology, Taiwan

^2. Department of Civil Engineering, National Pingtung University of Science and Technology

D9305503@mail.ntust.edu.tw

Abstract: In deep excavation, diaphragm wall deflection is an important field measurement that directly affects construction performance, site/adjacent building safety and project risk management. This paper applies historical data to forecast diaphragm wall deflection and proposes a new methodology, the “Evolutionary Fuzzy Neural Wall Deflection Prediction System”, to predict deep excavation wall deformation. At the core of this system is the Evolutionary Fuzzy Neural Inference Model (EFNIM), which joins together Genetic Algorithms (GAs), Fuzzy Logic (FL) and Neural Networks (NNs). This research established a historical database of wall deflection statistics from prior projects. The FL reasoning process and NN learning mechanism were then used to generalize a fuzzy rule. Finally, GAs were applied to optimize both FL’s and NN’s parameters coincidence. By inputting monitored wall deflection data from preceding deep excavation stages, the system developed in this paper helps users predict wall deformation in the upcoming stage and determine whether maximum allowable deflection has been exceeded. Simulation results demonstrated that past project data and experience can be utilized to predict wall deformation with a high level of precision in new projects.

[Min-Yuan Cheng, Yu-Wei Wu, Hsing-Chih Tsai, Chien-Ho Ko, Yen-Chuan Lin. Prediction of Diaphragm Wall Deflection in Deep Excavation Using Evolutionary Fuzzy Neural Inference Model. Life Sci J 2022;19(4):13-21]. ISSN 1097-8135 (print); ISSN 2372-613X (online). http://www.lifesciencesite.com. 3. doi:10.7537/marslsj190422.03.   

Keywords: Fuzzy Logic; Neural Networks; Genetic Algorithms; Diaphragm Wall; Excavation; Deflection

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Is There a Correlation between hs-CRP Levels and Functional Outcome of Ischemic Stroke?

Aliakbar Taheraghdam ¹, Siamak Aminnejad ², Ali Pashapour ¹, Reza Rikhtegar ¹, Kamyar Ghabili ³

^1. Neuroscience Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

^2. Students’ Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran

^3. Physical Medicine and Rehabilitation Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

reza_rikhtegar@yahoo.com

Abstract: C-reactive protein, a well-known marker of inflammation is being investigated as a probable marker of predicting acute cardiovascular events and its severity. The aim of the present study was to assess the possible role of highly-sensitivity C-reactive protein (hs-CRP) in predicting short-term functional outcome of ischemic stroke. A prospective study was conducted on subjects admitted with first attack of confirmed ischemic stroke. Serum hs-CRP was measured in the 2nd (CRP-D2) and 5th days (CRP-D5) post-stroke. Modified Rankin scale (MRS) was measured in all subjects in the 2nd (MRS-D2), 5th days (MRS-D5) and also 3 month (MRS-M3) after stroke to assess the short-term functional outcome and mortality of subjects. The mean age of the patients was 71.75±11.44 years. The mortality rate was 47.1% in the third months after stroke. There was no significant correlation between CRP-D2 and MRS-M3 and also between CRP-D5 and MRS-M3 (P>0.05). However there was a significant association between high CRP-D2 (CRP>3) and MRS-M3 and also between high CRP-D5 and MRS-M3 (P<0.005). This study showed that the value of CRP by itself could not predict the severity of short-term functional disability and it might not be useful as a clinical tool for predicting outcome.

[Taheraghdam A, Aminnejad S, Pashapour A, Rikhtegar R, Ghabili K. Is There a Correlation between hs-CRP Levels and Functional Outcome of Ischemic Stroke? Life Sci J 2022;19(4):22-26]. ISSN 1097-8135 (print); ISSN 2372-613X (online). http://www.lifesciencesite.com. 4. doi:10.7537/marslsj190422.04.   

Keywords: Atherosclerosis; C-reactive protein; functional outcome; inflammation; ischemic stroke; modified Rankin scale

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Effects of epidural injection of glucocorticoid and its combination with Bupivacaine in palliating chronic low back pain due to discopathy

Mohammad Taghi Mortazavi¹, Naghi Abedini¹, Iraj Lotfinia², Abdorrahim Afkhamzadeh³, Ali Delpisheh⁴, Reza Janmardi¹*, Samad EJ Golzari^5,6, Farhad Mirzaei², Majed Mashhouri¹

1. Anesthesiology Dept. Tabriz University of Medical Sciences, Tabriz, Iran

2. Neurosergery Dept. Tabriz University of Medical Sciences, Tabriz, Iran

3. Community Medicine Dept. Kurdistan University of Medical Sciences, Kurdistan Social Determinants of Health Research Center, Sanandaj, Iran

4. Clinical Epidemiology Dept. Ilam University of Medical Sciences, Ilam, Iran

5. Cardiovascular Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

6. Students Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran

Reza_janmardi@yahoo.com

Abstract: Background and Objectives: Chronic low back pain is defined as consistent or pendulous pain over 3 months. Epidural steroid injections (ESI) are common in treatment of chronic back pain. The present study was aimed to investigate the effects of epidural injection of glucocorticoid and bupivacaine compared to glucocorticoid alone in relieving chronic back pain due to discopathy. Materials and Methods: A randomized clinical trial was performed in the Shohada Medical Educational Center, Tabriz, Iran. Patients with chronic back pain who were candidates for epidural drug injection were recruited. They were divided into two groups of steroids or steroid and bupivacaine. Pain intensity, Oswestry Disability Index (ODI), Straight Leg Rising (SLR) test as well as clinical variables were evaluated before treatment and 3th month thereafter. Results: Overall, 17 males and 23 females with a mean age ± SD of 47.54 ± 12.11 years were enrolled in two equal groups. No significant difference was observed between two groups in terms of gender and body mass index. In both groups, a significant relationship was observed for ODI (p =0.001), pain intensity (p =0.001), and SLR test (p=0.001) before and after treatment. However, the corresponding association was not observed for ODI, pain intensity and SLR test (p>0.05).Conclusions: Epidural steroid injections either alone or combined with Bupivacaine with no priority are effectively relief chronic low back pain due to discopathy.

[Mortazavi M, Abedini N, Lotfinia I, Afkhamzadeh A, Delpisheh A, Janmardi R, Mirzaei F, Mashhouri M. Effects of epidural injection of glucocorticoid and its combination with Bupivacaine in palliating chronic low back pain due to discopathy. Life Sci J 2022;19(4):27-33]. ISSN 1097-8135 (print); ISSN 2372-613X (online). http://www.lifesciencesite.com. 5. doi:10.7537/marslsj190422.05.   

Keywords: Steroids, Bupivacaine, chronic low back pain

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Treatment of Acne vulgaris with Adaplane-Benzoyl Peroxide Topical Gel Formulation and Control and the Effects of Treatment Psychology

Monireh Saeidpour

Faculty of Pharmacy, Near East University, 99138 Nicosia, TRNC, Mersin 10,Turkey.

monirehsaeidpour@gmail.com

Abstract: Binary vulgaris is a very common disease among adolescents. Due to the effects it has on people's faces, it can impose many social and psychological costs on patients, so the treatment of this disease in addition to a health need is a social necessity. It is psychological. Therefore, in this study, Adapalene 0.1% and benzoyl peroxide 2.5% in a gel formulation were formulated for the first time in IRAN to guarantee better results and increase patients' acceptance of these two drugs at the same time. In the first stage, several formulations will be prepared using various jellies, which contain both benzoyl peroxide and adapalene, as well as other compounds such as hemoquantum, protective, etc. In the second stage, the various formulations prepared will be tested for physicochemical control. Control tests include determining the amount of active ingredient, checking for release simultaneously with the manufacture of the products, accelerated stability tests will be performed on the formulation to select the desired formulation based on the results of the control tests. In this work, we made eighty formulations, of which three formulations had the best physical and functional stability. Among them, la formulation was determined as the superior formulation and various physicochemical and microbial tests were performed on this formulation, all of which will confirm the superior product production method.

[Monireh Saeidpour. Treatment of Acne vulgaris with Adaplane-Benzoyl Peroxide Topical Gel Formulation and Control and the Effects of Treatment Psychology. Life Sci J 2022;19(4):34-44]. ISSN 1097-8135 (print); ISSN 2372-613X (online). http://www.lifesciencesite.com. 6. doi:10.7537/marslsj190422.06.   

Keywords: Acne treatment, adapalene topical gel control, benzoyl peroxide, vulgaris

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Transgenic crops and sterile insect releases are eco-friendly approaches for the management of major insect pests of crops

Mahwish Mustafa^1*, Abdul Rehman Bilal^2*, Muhammad Ameer Hamza³, Ali Hamid⁴, Muhammad Ijaz³, Syed Ghilman Mahdi Sherazi⁵, Hamza Rashid²

¹Center of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan

²Department of Entomology, University of Agriculture, Faisalabad, Sub campus Depalpur Okara, Pakistan

³Department of Plant Pathology, University of Agriculture, Sub campus Depalpur Okara,, Pakistan

⁴Department of Plant Breeding and Genetics, University of Agriculture, Sub campus Depalpur Okara, Pakistan

⁵Department of Agricultural Extension, University of agriculture Faisalabad sub campus Depalpur Okara, Pakistan

*Corresponding Email: mahwishmustfa@gmail.com; arrehman490@gmail.com

Abstract: Transgenic crops, genetically engineered to express insecticidal crystal (Cry) proteins derived from the bacterium Bacillus thuringiensis (Bt), have revolutionised agricultural pest insect control and are now planted on more than a billion acres worldwide. The benefits of this method would be lost if resistance to the toxins spread to a significant proportion of the pest population. Classical biological control is a pest control tool involving the release of imported natural enemies. The sterile insect technique is a pest control strategy used to suppress or eliminate regional populations of insects that pose significant threats to agriculture or human health. The process involves mass-rearing, sterilization and release of male insects who fail to produce viable offspring when they mate with wild females, which leads to a population decline. The SIT has constantly proven its capacity to suppress, contain, prevent (re)introduction or even locally eradicate populations of selected key insect plant and livestock pests. However, there is also a continuous need to further refine and improve the efficiency as well as the cost-effectiveness.

[Mahwish Mustafa, Abdul Rehman Bilal, Muhammad Ameer Hamza, Ali Hamid, Muhammad Ijaz, Syed Ghilman Mahdi Sherazi, Hamza Rashid. Transgenic crops and sterile insect releases are eco-friendly approaches for the management of major insect pests of crops. Life Sci J 2022;19(4):45-51]. ISSN 1097-8135 (print); ISSN 2372-613X (online). http://www.lifesciencesite.com. 7. doi:10.7537/marslsj190422.07. 

Key words: Transgenic crops, Integrated pest management (IPM), sterile insect technique (SIT)

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Life Sci J 2022;19(4):52-71]. ISSN 1097-8135 (print); ISSN 2372-613X (online). http://www.lifesciencesite.com. 8.

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Feasibility of serum liver enzymes as markers of microcystin-LR-induced liver injury：A systematic review and meta-analysis

Yu Fu ^1,2#，Yao Guo^1#, Zhihui Tian¹, Zhenxing Mao¹*

1. Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University,  Zhengzhou, 450001, Henan, PR China.

2. Department of Pharmacy, Henan Cancer Hospital, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, 450003, Henan, PR China.

Abstract: Microcystin-LR (MC-LR) is a hepatotoxin that can cause liver damage, resulting in overflow of liver enzymes from cells into the serum. However, it is still controversial to consider changes of liver enzymes in serum as biomarkers for the diagnosis of MC-LR-induced liver injury. Therefore, this study aimed to quantitatively evaluate the effects of MC-LR on liver biochemical markers in mice through a systematic review and meta-analysis. This will be used to clarify the feasibility of serum liver enzymes as biomarkers of MC-LR-induced liver injury. Literature was screened using the PRISMA process in the Scopus, Web of Science, and PubMed databases. The Cochrane Collaboration Tool was used to evaluate the quality of the publication. And the data was analyzed with Review Manager 5.3 and Stata 12.0. Four studies with 143 subjects were included from the 3819 identified papers. The analysis results showed that serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) concentrations were significantly increased at the oral dose of 1/2-1LD₅₀ of MC-LR. Subgroup analysis showed that, compared to the low-dose (1/4-1/2LD₅₀) group, serum ALT, AST and lactate dehydrogenase (LDH) concentrations were significantly increased in the high-dose (1/2-1LD₅₀) group of MC-LR exposure with the intraperitoneal injection. The above biochemical indexes were significantly increased several times compared with the control group. MC-LR can increase serum LDH, ALT and AST levels, and a clear dose-response relationship was observed after intraperitoneal injection of MC-LR. These results suggest that ALT, AST and LDH levels in serum are feasible as biomarkers of MC-LR-induced liver injury, which is helpful for early clinical diagnosis and prevention of MC-LR toxicity.

[Fu Y,Guo Y, Tian ZH, Mao ZX. Feasibility of serum liver enzymes as markers of microcystin-LR-induced liver injury：A systematic review and meta-analysis. Life Sci J 2022;19(4):72-82]. ISSN 1097-8135 (print); ISSN 2372-613X (online). http://www.lifesciencesite.com. 9. doi:10.7537/marslsj190422.09.

Keywords: Microcystin-LR; hepatic injury; alanine aminotransferase; aminotransferase; lactate dehydrogenase; meta-analysis  

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